Part:BBa_K3610036:Design
BAK1 ectodomain / mCherry N-terminal - for C. reinhardtii
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 925 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 855
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The in-frame translation of both BAK1 and GFP required choosing the scars carefully, including the 15 amino acid linker.
Source
BBa_K640001, BBa_K213601, BBa_K3610012, BBa_K3610004, BBa_K416001, BBa_K1150016, BBa_K3610018, BBa_K2984076
References
Ranf, Stefanie (2018): Pattern Recognition Receptors—Versatile Genetic Tools for Engineering Broad-Spectrum Disease Resistance in Crops. In: Agronomy 8, S. 134. DOI: 10.3390/agronomy8080134.
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.